You have full access to this article via your institution. So the question remains "What are the essential prerequisites for establishing a quantitative RT-PCR assay?" To date, a great deal of effort ...

Figure 1: Description and proof of principle for balanced PCR. As an alternative to the separation PCR step, LN1- and LN2-ligated plasmid DNA were mixed in different proportions and used in a ...

Quantitative polymerase chain reaction, or "qPCR", is considered the “second generation” of PCR and was first described in 1992. The past three decades have seen significant advances in qPCR ...

What is real-time quantitative PCR? qPCR uses one of two methods to detect the changes to DNA amplification: use of fluorescent dyes or sequence-specific DNA probes. qPCR is normally performed in a ...

SalivaDirect, an open-source protocol, avoids many of the supply bottlenecks of other tools and could be offered for as little as $10 a test. In this webinar, Evelien Van Hamme and Elina Kuznecova ...

Quantitative PCR (qPCR) and digital PCR (dPCR) are powerful tools for nucleic acid detection and quantification, each with distinct strengths. Both technologies play vital roles in genomics research, ...

In the six decades since Watson and Crick identified the double helical structure of DNA, a series of elegant and powerful experiments has established the broad outlines of how genetic information is ...

The series of thermoelectric coolers (TECs) is engineered specifically for demanding PCR (Polymerase Chain Reaction) testing applications, delivering high reliability, precise temperature control, and ...

PCR is a technique used to amplify target DNA in a sample. It’s a well-known method that has undergone numerous modifications to enhance its capabilities. This year, it’s turning 40 years old. PCR has ...